Demo input and outputTest run on bacterial genome:
What does this server do?
PCRTiler is used to generate multiple, specific and physically similar PCR oligos for tiled analysis at a genomic locus. Given a sequence (or multiple sequences), this server will then:
- Split your region(s) of interest in multiple segments according to your specifications;
- Use Primer3 to design multiple primer pairs targeting each of those segments;
- Use BlastN to discard primer pairs with cross-hybridization potential. You may choose to skip this step;
- Generate a report recommending oligos for PCR tiling analysis.
NEW: a MacOSX package of the standalone application is now available from the download page.